The PyroGene Recombinant Factor C (rFC) Assay is an advanced, endotoxin-specific fluorescent assay (0.005 – 5.0 EU/ml range) for detecting Gram-negative bacterial endotoxin.

Unlike traditional LAL, it uses a single enzymatic step where endotoxin activates recombinant Factor C to cleave a fluorogenic substrate, producing a measurable fluorescent signal.

Key advantages include eliminating false positives from glucans, predictable lot-to-lot performance, sustainable resource (no animal utilization), and FDA 510(k) approval as a final release test, establishing it as a reliable and equivalent alternative to LAL methods.