Orange Serum Agar (Dehydrated) is a specialized medium designed for isolating and enumerating spoilage microorganisms in acidic citrus products, such as lactic acid bacteria (Lactobacillus plantarum, L. brevis, Leuconostoc mesenteroides, L. dextranicum), yeasts, and molds. Its unique formulation, including orange serum, tryptone, glucose, and yeast extract, provides an optimal environment for these acid-tolerant organisms. Comparative studies have shown its superiority for total count and spoilage organism detection in citrus.